ARA-290

Nonerythropoietic Erythropoietin-Derived Tissue-Protective Peptide

ARA-290 is a synthetic, linear 11-amino-acid peptide derived from the three-dimensional helix-B surface region of erythropoietin. Also known as cibinetide or pyroglutamate helix-B surface peptide, it contains an N-terminal pyroglutamate residue and a free C-terminal carboxyl group.

OpenLabs supplies ARA-290 exclusively as a biochemical research material for controlled laboratory applications. It may be used in studies involving erythropoietin-derived signaling, inflammatory regulation, cytoprotection, peripheral nerve biology, cellular stress responses, and experimental tissue-repair pathways.

In experimental systems, ARA-290 has been investigated as a nonerythropoietic erythropoietin mimetic associated with signaling through an injury-responsive receptor system commonly described as the innate repair receptor. This proposed signaling complex involves the erythropoietin receptor and the beta-common receptor CD131, although its exact molecular organization and direct binding interactions remain under investigation. Reported downstream responses include modulation of JAK2, STAT, MAPK, and NF-kB-associated pathways, with effects that vary by cell type and experimental model.

Technical Information

Product name:

ARA-290

Alternative name:

Cibinetide; PHBSP; pHBSP; pyroglutamate helix-B surface peptide

Chemical class:

Synthetic linear oligopeptide; nonerythropoietic erythropoietin-derived peptide mimetic

Primary research target:

Proposed innate repair receptor signaling system involving EPOR and beta-common receptor CD131

Peptide length:

11 amino-acid residues

Molecular formula:

C₅₁H₈₄N₁₆O₂₁ (free peptide)

Molecular weight:

1257.31 g/mol (average molecular mass, free peptide)

CAS number:

1208243-50-8

PubChem CID:

91810664

Amino-Acid Sequence

pGlu-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser-OH

One-letter notation: XEQLERALNSS, where X represents pyroglutamate.

Scientific Background

ARA-290 was rationally designed from a solvent-exposed region of helix B in erythropoietin. Erythropoietin is best known for stimulating erythrocyte production through the classical homodimeric erythropoietin receptor. ARA-290 was developed to reproduce selected nonhematopoietic, tissue-protective signaling properties associated with erythropoietin while avoiding significant activation of the classical erythropoietic pathway. Experimental studies have therefore used ARA-290 to distinguish tissue-protective and inflammatory signaling from erythrocyte-producing activity.

Published experimental studies have examined ARA-290 in models of peripheral nerve injury, neuropathic allodynia, microglial activation, inflammatory tissue damage, ischemia-reperfusion injury, metabolic dysfunction, wound repair, retinal injury, and cardiovascular or renal stress. Rodent studies have reported changes in mechanical and thermal allodynia, spinal microglial activation, inflammatory mediator production, and tissue-injury measurements. Exploratory clinical investigations have also studied cibinetide in small-fiber neuropathy associated with sarcoidosis or type 2 diabetes and have evaluated corneal nerve-fiber measurements as experimental biomarkers. These findings remain investigational and do not establish an approved therapeutic use.

Potential Research Applications

• Characterization of proposed EPOR/CD131-associated innate repair receptor signaling and downstream JAK2, STAT, MAPK, or NF-kB pathways
• Investigation of nonerythropoietic signaling produced by erythropoietin-derived peptide mimetics
• Peripheral nerve injury, small-fiber neurobiology, neuroinflammation, and experimental neuropathic pain models
• Macrophage, microglial, cytokine, and innate immune-response studies
• Experimental cytoprotection, ischemia-reperfusion, metabolic, microvascular, wound-healing, retinal, cardiovascular, and renal injury models

Product Specifications

Supplied form:

Lyophilized peptide powder

Nominal quantity:

As stated on the vial label and lot-specific Certificate of Analysis

Purity:

Lot-specific; reported on the Certificate of Analysis

Identity verification:

Lot-specific analytical verification as reported on the Certificate of Analysis

Storage conditions:

Store unopened lyophilized material frozen, dry, tightly sealed, and protected from light. Follow the lot-specific Certificate of Analysis and validated laboratory stability procedures as the controlling storage instructions.

Certificate of Analysis:

Available for each lot